Whole genome sequencing with small amount of starting material

Model Organism: Worms Methods & Reagents
1

I am interested in sequencing the genome of a sterile mutant. The key difficulty with this approach is that I cannot easily isolate large quantities of starting material since I will have to pick the steriles manually, and I cannot just rinse off a few plates of worms. Can anyone point me in the right direction of a worm protocol for whole genome sequencing starting with small amounts of starting material?
Thanks,
Sven

2

TruSeq DNA sample prep kit (Nano) from Illimuna may help you to prepare DNA library from small amount of gDNA (100ng gDNA). Hope this helps.

3

Illumina’s Nextera kits go pretty low (50ng by protocol) and are super easy.
Probably you can go down to picogram levels
http://genome.cshlp.org/content/early/2014/07/30/gr.177881.114.full.pdf

4

We’ve made sequencing libraries from as few as 50 handpicked sterile adults using the NEBNext Ultra DNA kit (as little as 5 ng input gDNA) or the TruSeq ChIP kit (low ng input). PM if you’d like a detailed protocol for recovering sheared gDNA from small numbers of worms.