I heard Buck Samuels talk at the TAGC about a mixture of 12 bacterial strains they came up with based on sequencing the microbiome of C. elegans in nature. Apparently OP50 is not at all what the worms normally have in their gut and having a closer to natural microbiome improves their health. The paper is here: https://www.biorxiv.org/content/10.1101/2020.04.22.055426v1.full. They deposited the 12 bacterial strains at the CGC. However, it isn’t clear to me what is the best practice for using those 12 strains. Has anyone switched from OP50 to CeMBio? How are you doing it? Do you grow 12 flasks of bacteria and then mix them before plating? Grow them as a mixture? at what ratios? See any difference in the behavior of your worms in experiments?
In the paper they have seq. innoculum, I think the ration of bacteria in innoculum is actual ration that they put on the plate. However it is not clear how they grow it and when mixed