Is there a optimized Cerulean vector available for worms (codon usage, addition of introns)? If not, does anyone have any experience using a non-optimized Cerulean? Thanks, Steve
We made cerulean expression vectors (as well as mGFP, venus and cherry, N- and C- terminal fusion) with Pie-1 promoter elements for expression in the germline and embryo. I believe it was codon optimized with introns, but I’ll have to double check with Kraig Kumfer in the lab.
edit: they are “worm optimized”