I have been having this problem where worms seem not to like the entire plate and always stay around the edges of the plate where the bacteria is less and eaten very fast due to all worms hanging around there.
Sometimes, all of a sudden, I come back the next day to find most worm dead. Other times, no worm is dead, but just around the edges.
I got rid of all old OP50 batches, made fresh ones. No worms are dying, but just around the edges. I even put streptomycin in one batch, and another without. I still see this happening (hanging around the edges).
Any suggestions on what I can do. Because my worms never used to do that and have been doing that for around 2 months.
PS: it is not limited to N2, I have tried with around 10 strains.
Note: I do notice that my OP50 are growing faster when I incubate them. Is it possible that the bacterial lawn is so thick/too concentrated for my worms?
Thanks a lot!